Direct Enzymatic Assay for Alcohol Oxidase, Alcohol Dehydrogenase, and Formaldehyde Dehydrogenase in Colonies of Hansenula polymorpha
نویسندگان
چکیده
منابع مشابه
Bioconversion of airborne formaldehyde by immobilized formaldehyde dehydrogenase from the recombinant methylotrophic yeast Hansenula polymorpha
Formaldehyde (FA) is a very toxic pollutant. FA in indoor air has a negative effect on human health and should be removed by ventilation or by conversion to non-toxic products. The formaldehyde dehydrogenase (FdDH), a NADand glutathione-dependent enzyme from recombinant methylotrophic yeast Hansenula polymorpha, was tested for its ability to oxidize airborne FA. A continuous fluidized bed biore...
متن کاملMolecular characterization of the Hansenula polymorpha FLDI gene encoding formaldehyde dehydrogenase
Richard J. S. Baerends,{, Grietje J. Sulter, Thomas W. Jeffries, James M. Cregg and Marten Veenhuis* 1 Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands 2 Institute for Microbial and Biochemical Technology, Forest Products Laboratory, US Department of Agriculture, One Gifford Pinchot Drive, ...
متن کاملMolecular characterization of the Hansenula polymorpha FLD1 gene encoding formaldehyde dehydrogenase.
Glutathione-dependent formaldehyde dehydrogenase (FLD) is a key enzyme required forthe catabolism of methanol as a carbon source and certain primary amines, such as methylamine as nitrogen sources in methylotrophic yeasts. Here we describe the molecular characterization of the FLD1 gene from the yeast Hansenula polymorpha. Unlike the recently described Pichia pastoris homologue, the H. polymorp...
متن کاملThe dismutation of formaldehyde by liver alcohol dehydrogenase.
The results obtained led the authors to the suggestion that Reactions 2 and 4 are catalyzed by liver alcohol dehydrogenase but that Reaction 1 is due to an aldehyde dehydrogenase present in their liver preparation. Because of the inhomogeneity of the enzyme preparation employed, a reinvestigation of this problem with more highly purified enzyme was suggested. With the use of crystalline horse l...
متن کاملIsolation of mutants of Hansenula polymorpha defective in the assembly of octameric alcohol oxidase.
Alcohol oxidase (AO) is a peroxisomal enzyme that catalyses the first step in methanol metabolism in yeast. Monomeric, inactive AO protein is synthesised in the cytosol and subsequently imported into peroxisomes, where the enzymatically active, homo-octameric form is found. The mechanisms involved in AO octamer assembly are largely unclear. Here we describe the isolation of Hansenula polymorpha...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1980
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.39.1.268-269.1980